Hepatocyte Growth Factor (HGF),also known as hepatopoietin-A and scatter factor, is a pleiotropic mitogen belonging to the peptidase S1 family (plasminogen subfamily).

產品型號：Z03229

更新時間：2025-11-06

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產品介紹

產品簡介

Species

Human

Protein Construction

HGF (Gln32-Ser728)
Accession # P14210

Purity

> 95% as analyzed by SDS-PAGE

Endotoxin Level

< 0.2 EU/μg of protein by gel clotting method

Biological Activity

ED50 < 10.0 ng/ml, measured in a cell proliferation assay using 4MBr5 cells, corresponding to a specific activity of > 1.0 × 105 U/mg.

Expression System

CHO

Apparent Molecular Weight

88~90 kDa (single chain), 59~61 kDa (alpha chain), 30~34 kDa (beta chain), on SDS-PAGE under reducing conditions.

Formulation

Lyophilized after extensive dialysis against PBS.

Reconstitution

It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH?O or PBS up to 100 μg/ml.

Storage & Stability

Upon receiving, this product remains stable for 6 months at lower than -70°C. Upon reconstitution, the product should be stable for 1 week at 4°C or for 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

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