Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in

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產(chǎn)品介紹

產(chǎn)品簡(jiǎn)介

Species

Human

Protein Construction

IL-12 p35 (Arg23-Ser219)
Accession # P29459

IL-12 p40 (Ile23-Ser328)
Accession # P29460

Purity

> 95% as analyzed by SDS-PAGE
> 95% as analyzed by HPLC

Endotoxin Level

< 0.2 EU/μg of protein by gel clotting method

Biological Activity

ED50 < 2.0 ng/ml, measured by the induction of IFN-γ from NK cells, corresponding to a specific activity of > 5.0 × 105 U/mg.

Expression System

HEK 293

Theoretical Molecular Weight

75 kDa

Formulation

Lyophilized after extensive dialysis against PBS.

Reconstitution

It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH?O or PBS up to 100 μg/ml.

Storage & Stability

Upon receiving, this product remains stable for 6 months at lower than -70°C. Upon reconstitution, the product should be stable for 1 week at 4°C or for 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

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