GenScript cAMP ELISA Detection Kit is a competition enzyme-linked immunoassay which can be used for quantitative detection of cAMP in samples such as serum, plasma, saliva, cell culture supernatant, a

產品型號：L00460

更新時間：2025-11-06

廠商性質：代理商

訪問量：197

服務熱線

0592-5771844

立即咨詢

產品分類

PRODUCT CLASSIFICATION

免疫學

Western Blot產品蛋白純化 ELISA試劑盒抗體內毒素檢測和去除

查看全部產品

學會這幾招，ExCell Bio澳洲胎牛血清FND500不再難以選擇

干細胞無血清培養基：開啟干細胞研究與應用新未來

探索T細胞激活磁珠在免疫治療中的前景

eBlot L1快速濕轉儀優勢特點眾多，你不可錯過！

胎牛血清在細胞培養中的應用與優化技術

產品介紹

產品簡介

Product Description

Adenosine 3’, 5’ cyclic monophosphate (cAMP) is an important secondary messenger in signal transduction pathways that follows a number of extracellular signals. The G-Protein Coupled Receptors (GPCRs) are critical cell surface receptors that can be activated by different ligands, such as neurotransmitters, hormones and ions. The binding of the ligand to its receptor activates the effector adenylyl cyclase generating cAMP product. cAMP activates or inhibits various enzymes by promoting their phosphorylation or dephosphorylation. Its concentration is converted from adenosine triphosphate (ATP) via adenylyl cyclases (AC), and is inactivated by hydrolysis to 5′-AMP by the actions of phosphodiesterases.
GenScript cAMP ELISA Detection Kit is a competition enzyme-linked immunoassay which can be used for quantitative detection of cAMP in samples such as serum, plasma, saliva, cell culture supernatant, and urine. The anti-IgG Capture Plate is pre-coated with a fixed amount of Goat anti-mouse IgG to capture Mouse Anti-cAMP Monoclonal Antibody. When free cAMP or specimen and HRP-cAMP are added to the well, they compete in the solution to interact with the cAMP antibody captured on the plate. Other unbound molecules are removed by the wash step. The cAMP-HRP reacts with TMB Solution to develop a blue product in the solution. The reaction is stopped by adding stop solution and the color turns yellow which can be read at 450 nm by a Microtiter plate reader. Because the concentration of cAMP-HRP is held constant in the assay, the intensity of color is inversely proportional to the cAMP concentration in the sample and standards. Using the standard curve, the cAMP amount present in the unknown samples can be calculated by transforming its absorbance value.

Detection Range

1 ~243 pmol/ml

Sensitivity

0.61 pmol/ml

Test Samples

Serum, plasma, saliva, cell culture supernatant, and urine

Conveniency

All reagents and buffers for cAMP test are provided
Complete the test within 3.5 hours

Storage

The unopened kit is stable for 12 months if stored at 2-8 °C, and the opened kit is stable for 2 weeks at 2-8 °C. Do not freeze the kit.

Kit Contents

Components	Quantity	Part.No
Anti-IgG Capture Plate	1 plate (8 wells x 12 strips)	460-80
Anti-cAMP mAb	12 ml	460-20
HRP-cAMP	6 ml	460-30
cAMP Standards (0, 1, 3, 9, 27, 81, 243 pmol/ml)	1.5 ml	460-11, 12, 13, 14, 15, 16,17
cAMP Standard Stock (10 nmol/ml)	500 μl	460-10
Assay Buffer A	60 ml	460-60
Assay Buffer B	1 ml	460-90
20× Wash Solution	40 ml	B-A1-70
TMB Solution	12 ml	B-A1-40
Stop Solution	6 ml	B-A1-50
Plate Sealer	2 pieces	N/A